Publication Date

2015

Document Type

Honors Thesis

Department

Biological Sciences

Keywords

Microscopy, Fluorescence microscopy, Saccharomyces cerevisiae-Genetics., Molecular biology, Dynein, Single molecule microscopy, S. cerevisiae, Fluorescence

Abstract

Microscopy has served as a crucial tool for the advancement of biological knowledge, such as discoveries of cellular and subcellular components and mechanisms, molecular structures and function, and protein interactions. Recently, there is an increasing demand for imaging individual cells and molecules, which has driven the development of new microscopy techniques focused on this purpose. Due to technical challenges, these techniques are mostly implemented in vitro. However, there is a push towards in vivo assays, despite potential difficulties. This project aims to exploit the genetic tractability of S. cerevisiae, a simple, eukaryotic model organism, in order to develop fluorescent assays that will improve single molecule microscopy imaging for biological studies in vivo, using dynein motor proteins as a test model.

Language

English

Comments

iii, 47 pages : illustrations (some color). Honors project-Smith College, 2015. Includes bibliographical references (pages 46-47)

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