Publication Date

2017-5

Document Type

Honors Thesis

Degree Name

Bachelor of Arts

Department

Biochemistry

Advisors

Nathan D. Derr

Keywords

GFP, Fluorescence microscopy, Yeast cell biology, S. cerevisiae, Fluorescence, Cre recombinase, Saccharomyces cerevisiae, Green fluorescent protein, Yeast-Cytology, Genetic recombination

Abstract

While there have been many advances in fluorescence microscopy for single molecules in recent years, there are still limitations to studying protein activity and localization over time through fluorescence. It is difficult to distinguish between closelypacked fluorescent molecules, and impossible to determine the difference between proteins expressed at different times, and therefore observe differences in behavior over time. There is a need for a method of fluorescence that enables the study of the interactions of proteins throughout their lifetimes. This project aims to construct a system in S. cerevisiae for the expression of multifluorescent protein tags that vary in fluorescence color over time, enabling the differentiation of proteins expressed at different times.

Language

English

Comments

47 pages : color illustrations. Includes bibliographical references (pages 45-47)

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