The formation of the central nervous system depends on the coordinated development of neural and glial cell types that arise from a common precursor. Using an existing group of zebrafish mutants generated by viral insertion, we performed a "shelf-screen" to identify genes necessary for astroglial development and axon scaffold formation. We screened 274 of 315 viral insertion lines using antibodies that label axons (anti-Acetylated Tubulin) and astroglia (anti-Gfap) and identified 25 mutants with defects in gliogenesis, glial patterning, neurogenesis, and axon guidance. We also identified a novel class of mutants affecting radial glial cell numbers. Defects in astroglial patterning were always associated with axon defects, supporting an important role for axon-glial interactions during axon scaffold development. The genes disrupted in these viral lines have all been identified, providing a powerful new resource for the study of axon guidance, glio- and neurogenesis, and neuron-glial interactions during development of the vertebrate CNS.
Astroglia, Axon guidance, Chiasm, Gfap, Glia, Gliogenesis, Hindbrain, Insertional mutants, Motor axon, Neurogenesis, Poc, Radial glia, Zebrafish
© 2010 Wiley-Liss, Inc.
Barresi, Michael J.F.; Burton, Sean; Dipietrantonio, Kristina; Amsterdam, Adam; Hopkins, Nancy; and Karlstrom, Rolf O., "Essential Genes for Astroglial Development and Axon Pathfinding During Zebrafish Embryogenesis" (2010). Biological Sciences: Faculty Publications, Smith College, Northampton, MA.