Document Type

Article

Publication Date

1-18-2018

Publication Title

PLOS Neglected Tropical Diseases

Abstract

Maximizing the recovery of PCR-amplifiable DNA is an important consideration in the optimization of field-ready PCR-based diagnostic techniques. Fecal specimen preservation is particularly important due to the universal use of stool for the non-invasive diagnosis of soil-transmitted helminth (STH) infections. A comprehensive and systematic assessment of methods for maintaining and preserving soil-transmitted helminth (STH) DNA, particularly from fragile eggs subjected to nuclease exposure in stool, has not been conducted previously. Through the comparative analysis of a variety of preservation techniques, the present study demonstrates that fecal samples designated for PCR-based molecular analysis maintain sample integrity for at least 60 days when stored at 4 ̊C. While the expedited establishment of a cold chain for stool sample storage remains the best-practice procedure for downstream molecular analysis, a variety of preservatives can facilitate the extended preservation of sample quality, even under unfavorable temperatures. Factors such as preservative cost, inhibitor resistance, toxicity, availability, associated labor, sample shipping requirements and study purpose should also be considered when determining an appropriate method for fecal specimen preservation. Taking all of these factors into consideration, the use of 95% ethanol as a preservative is recommended in most situations.

Volume

12

Issue

1

DOI

doi.org/10.1371/ journal.pntd.0006130

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Rights

© 2018 Papaiakovou et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Included in

Biology Commons

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.