This paper is the first molecular phylogenetic study on Brugia para- sites (family Onchocercidae) which includes 6 of the 10 species of this genus : B. beaveri Ash et Little, 1 9 6 4 ; B. buckleyiDissanaike et Paramananthan, 1961 ; B. malayi (Brug,1927) Buckley, 1960 ; B. pohangi, (Buckley et Edeson, 1956) Buckley, 1960; B. patei (Buckley, Nelson etHeisch,1958) Buckley, 1960 and B. limori Partono et al., 1 9 7 7 . Hha I repeat sequences are 322 nucleotides long, highly repeated, tandemly arranged and unique to the nuclear genomes of the genus Brugia. Hha I repeat sequence data was collected by PCR, cloning and dideoxy sequencing. The Hha I repeat sequences were aligned and analyzed by maximum parsimony algorithms, distance methods and maximum likelihood methods to construct phylogenetic trees. Bootstrap analysis was used to test the robustness of the different phylogenetic reconstructions. The data indicated that the Hha I repeat sequences are highly conserved within species yet differ significantly between species. The various tree-building methods gave identical results. Bootstrap analyses on the Hha I repeat sequence data set identified at least two clades : the B. pahangi- B. beaveri clade and the B. malayi-B.timori-B. buckleyi clade; the first clade includes parasites of carnivores from Asia and America; the second includes species from primates and lagomorphs from Asiatic region. It was also noted that the Hha I repeat sequences obtained from B. malayi were identical to those obtained from B.timori, indicating very recent speciation.
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Xie, Hong; Bain, O.; and Williams, Steven A., "Molecular Phylogenetic Studies of the Genus Brugia" (1994). Biological Sciences: Faculty Publications, Smith College, Northampton, MA.