To access this work you must either be on the Smith College campus OR have valid Smith login credentials.
On Campus users: To access this work if you are on campus please Select the Download button.
Off Campus users: To access this work from off campus, please select the Off-Campus button and enter your Smith username and password when prompted.
Non-Smith users: You may request this item through Interlibrary Loan at your own library.
Developing synthetic multi-fluorescence techniques in S. cerevisiae for single molecule microscopy
Microscopy, Fluorescence microscopy, Saccharomyces cerevisiae-Genetics., Molecular biology, Dynein, Single molecule microscopy, S. cerevisiae, Fluorescence
Microscopy has served as a crucial tool for the advancement of biological knowledge, such as discoveries of cellular and subcellular components and mechanisms, molecular structures and function, and protein interactions. Recently, there is an increasing demand for imaging individual cells and molecules, which has driven the development of new microscopy techniques focused on this purpose. Due to technical challenges, these techniques are mostly implemented in vitro. However, there is a push towards in vivo assays, despite potential difficulties. This project aims to exploit the genetic tractability of S. cerevisiae, a simple, eukaryotic model organism, in order to develop fluorescent assays that will improve single molecule microscopy imaging for biological studies in vivo, using dynein motor proteins as a test model.
Hocker, Malayna A., "Developing synthetic multi-fluorescence techniques in S. cerevisiae for single molecule microscopy" (2015). Honors Project, Smith College, Northampton, MA.
Off Campus Download
iii, 47 pages : illustrations (some color). Honors project-Smith College, 2015. Includes bibliographical references (pages 46-47)