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Stylianos P. Scordilis
Bachelor of Arts
Mice-Muscles, Proteomics, Myogenesis, Muscles, Satellite cells, C@C!@, Anabolic steroids, Anabolic-androgenic steroids, Nandrolone, Nandrolone decanoate, 19-nortestosteron, Skeletal muscle, Tandem mass tagging, Mass spectrometry
Little is known about the biochemical mechanisms by which anabolic-androgenic steroids produce physiological changes in skeletal muscle tissue. In this study, C2C12 cells were grown in three different media: control (CTRL), vehicle control (DMSO), and anabolic steroid (nandrolone decanoate) containing (NAND). Cells were then harvested at three time points that represent the three stages of myogenesis: myoblasts (Day 0), early myotubes (Day 5) and late myotubes (Day 9). Whole cell extracts at each stage in each treatment (n=3) were labeled using tandem mass-tags (TMT) and combined into four separate TMT multiplex replicates. High-pressure liquid-chromatography-coupled mass spectrometry was used to identify and quantify the proteins expressed in each condition. Proteins determined to be myogenically-regulated in previous comprehensive literature on satellite cell myogenesis (Le Bihan et al., 2015) were selected and categorized into euKaryotic Orthologous Groups (KOGs) for functional annotation and analysis (Huerta Capas et al., 2016). KOGs that were significantly affected by NAND treatment compared to CTRL and DMSO treatments were further analyzed at the individual protein level. Proteins involved in cell cycle control, replication, and proliferation were found to decrease expression in the presence of nandrolone at the earlier stages of myogenesis, while those involved with cytoskeletal structure and strength, extracellular structure, membrane biogenesis, signal transduction, and energy production were found to increase expression in the presence of nandrolone during myogenesis. These proteomic results correlate with the reported physiological hypertrophic effects of anabolic-androgenic steroids reported in in vivo usage, and shed light on the biochemical mechanisms by which these molecules induce changes at the physiological level.
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Morris, Emily Ann, "The effect of an anabolic steroid on the proteome of developing muscle cells" (2019). Honors Project, Smith College, Northampton, MA.
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