Publication Date

2019

Document Type

Honors Project

Degree Name

Bachelor of Arts

Department

Neuroscience

Advisors

David Bickar

Keywords

Dopamine, DOPAC, HVA, 3-MT, Saliva, Parkinson's disease, LC-ESI-MS/MS

Abstract

Given that submandibular glands are affected in the early stages of Parkinson’s disease (Ma, Gao, Li, Ma, & Feng, 2019) , identification and quantification of salivary dopamine and its metabolites, homovanillic acid, 3-methoxytyramine and 3,4-dihydroxyphenylacetic acid, can be used as a potential diagnostic tool to monitor the initiation and progression of the disease. A sensitive assay method was developed for the determination of dopamine and metabolites through LC-ESI-MS. Although further optimization of the protocol is required for the quantification of these analytes in human salivary samples, the postulated method has laid the foundation for the collection, purification and identification of dopamine and its metabolites as potential biomarkers for Parkinson’s disease.

Rights

©2019 Josselyn Sofia Vergara Cobos. Access limited to the Smith College community and other researchers while on campus. Smith College community members also may access from off-campus using a Smith College log-in. Other off-campus researchers may request a copy through Interlibrary Loan for personal use.

Language

English

Comments

67, 8 pages : illustrations (some color). Includes bibliographical references (pages 50-66)

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