Document Type
Article
Publication Date
4-1-1998
Publication Title
Journal of Cell Science
Abstract
A myosin heavy chain polypeptide has been identified and localized in Nicotiana pollen tubes using monoclonal anti-myosin antibodies. The epitopes of these antibodies were found to reside on the myosin heavy chain head and rod portion and were, therefore, designated anti-S-1 (myosin S-1) and anti-LMM (light meromyosin). On Western blots of the total soluble pollen tube proteins, both anti-S-1 and anti-LMM label a polypeptide of approximately 175,000 Mr. Immunofluorescence microscopy shows that both antibodies yield numerous fluorescent spots throughout the whole length of the tube, often with an enrichment in the tube tip. These fluorescent spots are thought to represent vesicles and/or organelles in the pollen tubes. In addition to this common pattern, anti-S-1 stains both the generative cell and the vegetative nuclear envelope. The different staining patterns of the nucleus between anti-S-1 and anti-LMM may be caused by some organization and/or anchorage state of the myosin molecules on the nuclear surface that differs from those on the vesicles and/or organelles.
Keywords
myosin, Western blotting, immunofluorescence, cytoplasmic streaming, nuclear migration
Volume
92
Issue
4
First Page
569
Last Page
574
DOI
doi.org/10.1242/jcs.92.4.569
Recommended Citation
Tang, X. J.; Hepler, P. K.; and Scordilis, Stylianos P., "Immunochemical and Immunocytochemical Identification of a Myosin Heavy Chain Polypeptide in Nicotiana Pollen Tubes" (1998). Biological Sciences: Faculty Publications, Smith College, Northampton, MA.
https://scholarworks.smith.edu/bio_facpubs/148
Comments
Archived as published.
Open access article.