Radial Glia Inhibit Peripheral Glial Infiltration into the Spinal Cord at Motor Exit Point Transition Zones

Authors

Cody J. Smith, University of Virginia
Kimberly Johnson, Smith College
Taylor G. Welsh, University of Virginia
Michael J.F. Barresi, Smith CollegeFollow
Sarah Kucenas, University of Virginia

Document Type

Article

Publication Date

7-1-2016

Publication Title

GLIA

Abstract

In the mature vertebrate nervous system, central and peripheral nervous system (CNS and PNS, respectively) GLIA myelinate distinct motor axon domains at the motor exit point transition zone (MEP TZ). How these cells preferentially associate with and myelinate discrete, non-overlapping CNS versus PNS axonal segments, is unknown. Using in vivo imaging and genetic cell ablation in zebrafish, we demonstrate that radial glia restrict migration of PNS glia into the spinal cord during development. Prior to development of radial glial endfeet, peripheral cells freely migrate back and forth across the MEP TZ. However, upon maturation, peripherally located cells never enter the CNS. When we ablate radial glia, peripheral glia ectopically migrate into the spinal cord during developmental stages when they would normally be restricted. These findings demonstrate that radial glia contribute to both CNS and PNS development and control the unidirectional movement of glial cell types across the MEP TZ early in development.

Keywords

CNS/PNS transition zone, Motor exit point glia, Neural crest cells, Radial glia, Zebrafish

Volume

64

Issue

7

First Page

1138

Last Page

1153

DOI

10.1002/glia.22987

ISSN

08941491

Rights

© The authors

Comments

Peer reviewed accepted manuscript.

Recommended Citation

Smith, Cody J.; Johnson, Kimberly; Welsh, Taylor G.; Barresi, Michael J.F.; and Kucenas, Sarah, "Radial Glia Inhibit Peripheral Glial Infiltration into the Spinal Cord at Motor Exit Point Transition Zones" (2016). Biological Sciences: Faculty Publications, Smith College, Northampton, MA.
https://scholarworks.smith.edu/bio_facpubs/196