A DNA Origami Platform for Quantifying Protein Copy Number in Super-Resolution

Authors

Francesca Cella Zanacchi, Barcelona Institute of Science and Technology (BIST)
Carlo Manzo, Smith College
Angel S. Alvarez, Smith College
Nathan D. Derr, Smith CollegeFollow
Maria F. Garcia-Parajo, University of Pennsylvania Perelman School of Medicine
Melike Lakadamyali, Barcelona Institute of Science and Technology (BIST)

Document Type

Article

Publication Date

7-28-2017

Publication Title

Nature Methods

Abstract

Single-molecule-based super-resolution microscopy offers researchers a unique opportunity to quantify protein copy number with nanoscale resolution. However, while fluorescent proteins have been characterized for quantitative imaging using calibration standards, similar calibration tools for immunofluorescence with small organic fluorophores are lacking. Here we show that DNA origami, in combination with GFP antibodies, is a versatile platform for calibrating fluorophore and antibody labeling efficiency to quantify protein copy number in cellular contexts using super-resolution microscopy.

Volume

14

Issue

8

First Page

789

Last Page

792

DOI

10.1038/nmeth.4342

ISSN

15487091

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Rights

Licensed to Smith College and distributed CC-BY under the Smith College Faculty Open Access Policy.

Comments

Peer reviewed accepted manuscript.

Recommended Citation

Cella Zanacchi, Francesca; Manzo, Carlo; Alvarez, Angel S.; Derr, Nathan D.; Garcia-Parajo, Maria F.; and Lakadamyali, Melike, "A DNA Origami Platform for Quantifying Protein Copy Number in Super-Resolution" (2017). Biological Sciences: Faculty Publications, Smith College, Northampton, MA.
https://scholarworks.smith.edu/bio_facpubs/219