Combined Genome and Transcriptome Analyses of the Ciliate Schmidingerella arcuata (Spirotrichea) Reveal Patterns of DNA Elimination, Scrambling, and Inversion

Authors

Susan A. Smith, University of Connecticut Avery Point Campus
Xyrus X. Maurer-Alcalá, University of Bern
Ying Yan, Smith College
Laura A. Katz, Smith CollegeFollow
Luciana F. Santoferrara, University of Connecticut Avery Point Campus
George B. McManus, University of Connecticut Avery Point Campus

Document Type

Article

Publication Date

1-1-2020

Publication Title

Genome Biology and Evolution

Abstract

Schmidingerella arcuata is an ecologically important tintinnid ciliate that has long served as a model species in plankton trophic ecology. We present a partial micronuclear genome and macronuclear transcriptome resource for S. arcuata, acquired using single-cell techniques, and we report on pilot analyses including functional annotation and genome architecture. Our analysis shows major fragmentation, elimination, and scrambling in the micronuclear genome of S. arcuata. This work introduces a new nonmodel genome resource for the study of ciliate ecology and genomic biology and provides a detailed functional counterpart to ecological research on S. arcuata.

Keywords

Ciliophora, Genome architecture, Macronucleus, Micronucleus, Single-cell 'omics, Tintinnid

Volume

12

Issue

9

First Page

1616

Last Page

1622

DOI

10.1093/GBE/EVAA185

Rights

The Author(s) 2020.

Comments

Archived as published.

Recommended Citation

Smith, Susan A.; Maurer-Alcalá, Xyrus X.; Yan, Ying; Katz, Laura A.; Santoferrara, Luciana F.; and McManus, George B., "Combined Genome and Transcriptome Analyses of the Ciliate Schmidingerella arcuata (Spirotrichea) Reveal Patterns of DNA Elimination, Scrambling, and Inversion" (2020). Biological Sciences: Faculty Publications, Smith College, Northampton, MA.
https://scholarworks.smith.edu/bio_facpubs/80