Document Type
Article
Publication Date
1-1-2016
Publication Title
Physiological Reports
Abstract
Circulating angiogenic cells (CAC) influence vascular repair through the secretion of proangiogenic factors and cytokines. While CAC are deficient in patients with diabetes and exercise has a beneficial effect on CACs, the impact of these factors on paracrine secretion from CAC is unknown. We aimed to determine whether the in vitro secretion of selected cytokines and nitric oxide (NO) from CAC is influenced by hyperglycemia and acute exercise. Colonyforming unit CAC (CFU-CAC) were cultured from young active men (n = 9, 24 ± 2 years) at rest and after exercise under normal (5 mmol/L) and elevated (15 mmol/L) glucose. Preliminary relative multiplex cytokine analysis revealed that CAC conditioned culture media contained three of six measured cytokines: transforming growth factor-beta-1 (TGFβ1), tumor necrosis factor alpha (TNFα), and monocyte chemotactic protein-1 (MCP-1). Single quantitative cytokine analysis was used to determine the concentration of each cytokine from the four conditions. NO was measured via Griess assay. There was a significant effect of CAC exposure to in vivo exercise on in vitro TGFβ1 secretion (P = 0.024) that was independent of glucose concentration. There was no effect of glucose or acute exercise on TNFα or MCP-1 concentration (both P > 0.05). The concentration of NO from CFU-CAC cultured in elevated glucose was lower following acute exercise (P = 0.002) suggesting that exercise did not maintain NO secretion under hyperglycemic conditions. Our results identify paracrine signaling factors that may be responsible for the proangiogenic function of CFU-CAC and an influence of acute exercise and elevated glucose on CFU-CAC soluble factor secretion.
Keywords
Acute exercise, Circulating angiogenic cells, Cytokine, Diabetes, Inflammation, Nitric oxide
Volume
4
Issue
3
DOI
10.14814/phy2.12649
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Rights
© 2016 The Authors.
Recommended Citation
Witkowski, Sarah; Guhanarayan, Gayatri; and Burgess, Rachel, "Glucose and Acute Exercise Influence Factors Secreted by Circulating Angiogenic Cells in vitro" (2016). Exercise and Sport Studies: Faculty Publications, Smith College, Northampton, MA.
https://scholarworks.smith.edu/ess_facpubs/13
Comments
Archived as published.