PAR2 (protease-activated receptor 2) Deficiency Attenuates Atherosclerosis in Mice

Authors

Shannon M. Jones, University of Cincinnati College of Medicine
Adrien Mann, University of Cincinnati College of Medicine
Kelsey Conrad, University of Cincinnati College of MedicineFollow
Keith Saum, University of Cincinnati College of Medicine
David E. Hall, University of Cincinnati College of Medicine
Lisa M. McKinney, University of Cincinnati College of Medicine
Nathan Robbins, University of Cincinnati College of Medicine
Joel Thompson, University of Kentucky College of Medicine
Abigail D. Peairs, University of Cincinnati College of Medicine
Eric Camerer, PARCC - Paris-Centre de Recherche Cardiovasculaire
Katey J. Rayner, Institut de Cardiologie de l'Université d'Ottawa
Michael Tranter, University of Cincinnati College of Medicine
Nigel Mackman, UNC School of Medicine
A. Phillip Owens, University of Cincinnati College of Medicine

Document Type

Article

Publication Date

6-1-2018

Publication Title

Arteriosclerosis, Thrombosis, and Vascular Biology

Abstract

Objective-PAR2 (protease-activated receptor 2)-dependent signaling results in augmented inflammation and has been implicated in the pathogenesis of several autoimmune conditions. The objective of this study was to determine the effect of PAR2 deficiency on the development of atherosclerosis. Approach and Resutle-PAR2 mRNA and protein expression is increased in human carotid artery and mouse aortic arch atheroma versus control carotid and aortic arch arteries, respectively. To determine the effect of PAR2 deficiency on atherosclerosis, male and female low-density lipoprotein receptor-deficient (Ldlr-/-) mice (8-12 weeks old) that were Par2+/+ or Par2-/- were fed a fat-and cholesterol-enriched diet for 12 or 24 weeks. PAR2 deficiency attenuated atherosclerosis in the aortic sinus and aortic root after 12 and 24 weeks. PAR2 deficiency did not alter total plasma cholesterol concentrations or lipoprotein distributions. Bone marrow transplantation showed that PAR2 on nonhematopoietic cells contributed to atherosclerosis. PAR2 deficiency significantly attenuated levels of the chemokines Ccl2 and Cxcl1 in the circulation and macrophage content in atherosclerotic lesions. Mechanistic studies using isolated primary vascular smooth muscle cells showed that PAR2 deficiency is associated with reduced Ccl2 and Cxcl1 mRNA expression and protein release into the supernatant resulting in less monocyte migration.Conclusion-Our results indicate that PAR2 deficiency is associated with attenuation of atherosclerosis and may reduce lesion progression by blunting Ccl2-and Cxcl1-induced monocyte infiltration.

Keywords

Atherosclerosis, Cholesterol, Inflammation, Macrophage, Mice

Volume

38

Issue

6

First Page

1271

Last Page

1282

DOI

10.1161/ATVBAHA.117.310082

ISSN

10795642

Rights

© 2018 American Heart Association, Inc.

Comments

Archived as published.

Recommended Citation

Jones, Shannon M.; Mann, Adrien; Conrad, Kelsey; Saum, Keith; Hall, David E.; McKinney, Lisa M.; Robbins, Nathan; Thompson, Joel; Peairs, Abigail D.; Camerer, Eric; Rayner, Katey J.; Tranter, Michael; Mackman, Nigel; and Owens, A. Phillip, "PAR2 (protease-activated receptor 2) Deficiency Attenuates Atherosclerosis in Mice" (2018). Exercise and Sport Studies: Faculty Publications, Smith College, Northampton, MA.
https://scholarworks.smith.edu/ess_facpubs/50