Document Type
Article
Publication Date
7-18-2012
Publication Title
PLoS ONE
Abstract
Organ development leads to the emergence of organ function, which in turn can impact developmental processes. Here we show that fluid flow-induced collective epithelial migration during kidney nephron morphogenesis induces cell stretch that in turn signals epithelial proliferation. Increased cell proliferation was dependent on PI3K signaling. Inhibiting epithelial proliferation by blocking PI3K or CDK4/Cyclin D1 activity arrested cell migration prematurely and caused a marked overstretching of the distal nephron tubule. Computational modeling of the involved cell processes predicted major morphological and kinetic outcomes observed experimentally under a variety of conditions. Overall, our findings suggest that kidney development is a recursive process where emerging organ function "feeds back" to the developmental program to influence fundamental cellular events such as cell migration and proliferation, thus defining final organ morphology.
Volume
7
Issue
7
DOI
10.1371/journal.pone.0039992
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Rights
© 2012 Vasilyev et al.
Recommended Citation
Vasilyev, Aleksandr; Liu, Yan; Hellman, Nathan; Pathak, Narendra; and Drummond, Iain A., "Mechanical Stretch and PI3K Signaling Link Cell Migration and Proliferation to Coordinate Epithelial Tubule Morphogenesis in the Zebrafish Pronephros" (2012). Neuroscience: Faculty Publications, Smith College, Northampton, MA.
https://scholarworks.smith.edu/nsc_facpubs/35
Comments
Archived as published. Open access article.