Filopodial Calcium Transients Regulate Growth Cone Motility and Guidance through Local Activation of Calpain

Authors

Estuardo Robles, University of Wisconsin-MadisonFollow
Anna Huttenlocher, University of Wisconsin-Madison
Timothy M. Gomez, University of Wisconsin-Madison

Document Type

Article

Publication Date

5-22-2003

Publication Title

Neuron

Abstract

Spontaneous intracellular calcium ([Ca2+]i) transients in growth cone filopodia reduce filopodial motility, slow neurite outgrowth, and promote turning when generated asymmetrically; however, the downstream effectors of these Ca2+-dependent behaviors are unknown. We report that Ca2+ transients in filopodia activate the intracellular protease calpain, which slows neurite outgrowth and promotes repulsive growth cone turning upon local activation. Active calpain alters the balance between tyrosine kinase and phosphatase activities in filopodia, resulting in a net decrease in tyrosine phosphorylation, which mediates both filopodial stabilization and reduced lamellipodial protrusion. Our findings indicate that locally generated Ca2+ signals repel axon outgrowth through calpain-dependent regulation of phosphotyrosine signaling at integrin-mediated adhesion sites.

Volume

38

Issue

4

First Page

597

Last Page

609

DOI

10.1016/S0896-6273(03)00260-5

ISSN

08966273

Comments

Archived as published. Open access article.

Recommended Citation

Robles, Estuardo; Huttenlocher, Anna; and Gomez, Timothy M., "Filopodial Calcium Transients Regulate Growth Cone Motility and Guidance through Local Activation of Calpain" (2003). Neuroscience: Faculty Publications, Smith College, Northampton, MA.
https://scholarworks.smith.edu/nsc_facpubs/77