Deconstructing Circadian Disruption: Assessing the Contribution of Reduced Peripheral Oscillator Amplitude on Obesity and Glucose Intolerance in Mice

Authors

Vincent van der Vinne, University of Massachusetts Medical School
Blanca Martin Burgos, Smith College
Mary E. Harrington, Smith CollegeFollow
David R. Weaver, University of Massachusetts Medical School

Document Type

Article

Publication Date

3-25-2020

Publication Title

Journal of Pineal Research

Abstract

Disturbing the circadian regulation of physiology by disruption of the rhythmic environment is associated with adverse health outcomes but the underlying mechanisms are unknown. Here, the response of central and peripheral circadian clocks to an advance or delay of the light-dark cycle was determined in mice. This identified transient damping of peripheral clocks as a consequence of an advanced light-dark cycle. Similar depression of peripheral rhythm amplitude was observed in mice exposed to repeated phase shifts. To assess the metabolic consequences of such peripheral amplitude depression in isolation, temporally chimeric mice lacking a functional central clock (Vgat-Cre+ Bmal1fl/fl) were housed in the absence of environmental rhythmicity. In vivo PER2::LUC bioluminescence imaging of anesthetized and freely moving mice revealed that this resulted in a state of peripheral amplitude depression, similar in severity to that observed transiently following an advance of the light-dark cycle. Surprisingly, our mice did not show alterations in body mass or glucose tolerance in males or females on regular or high-fat diets. Overall, our results identify transient damping of peripheral rhythm amplitude as a consequence of exposure to an advanced light-dark cycle but chronic damping of peripheral clocks in isolation is insufficient to induce adverse metabolic outcomes in mice.

DOI

doi.org/10.1111/jpi.12654

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Rights

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2020 The Authors. Journal of Pineal Research published by John Wiley & Sons Ltd.

Comments

Archived as published.

Recommended Citation

van der Vinne, Vincent; Burgos, Blanca Martin; Harrington, Mary E.; and Weaver, David R., "Deconstructing Circadian Disruption: Assessing the Contribution of Reduced Peripheral Oscillator Amplitude on Obesity and Glucose Intolerance in Mice" (2020). Psychology: Faculty Publications, Smith College, Northampton, MA.
https://scholarworks.smith.edu/psy_facpubs/74