To access this work you must either be on the Smith College campus OR have valid Smith login credentials.
On Campus users: To access this work if you are on campus please Select the Download button.
Off Campus users: To access this work from off campus, please select the Off-Campus button and enter your Smith username and password when prompted.
Non-Smith users: You may request this item through Interlibrary Loan at your own library.
Publication Date
2017-5
First Advisor
Nathan D. Derr
Document Type
Honors Project
Degree Name
Bachelor of Arts
Department
Biochemistry
Keywords
GFP, Fluorescence microscopy, Yeast cell biology, S. cerevisiae, Fluorescence, Cre recombinase, Saccharomyces cerevisiae, Green fluorescent protein, Yeast-Cytology, Genetic recombination
Abstract
While there have been many advances in fluorescence microscopy for single molecules in recent years, there are still limitations to studying protein activity and localization over time through fluorescence. It is difficult to distinguish between closelypacked fluorescent molecules, and impossible to determine the difference between proteins expressed at different times, and therefore observe differences in behavior over time. There is a need for a method of fluorescence that enables the study of the interactions of proteins throughout their lifetimes. This project aims to construct a system in S. cerevisiae for the expression of multifluorescent protein tags that vary in fluorescence color over time, enabling the differentiation of proteins expressed at different times.
Language
English
Recommended Citation
Knobloch, Emmie Marie, "Clocking multifluorescent protein expression in yeast" (2017). Honors Project, Smith College, Northampton, MA.
https://scholarworks.smith.edu/theses/1830
Smith Only:
Off Campus Download
Comments
47 pages : color illustrations. Includes bibliographical references (pages 45-47)