Bachelor of Arts
Nathan D. Derr
GFP, Fluorescence microscopy, Yeast cell biology, S. cerevisiae, Fluorescence, Cre recombinase, Saccharomyces cerevisiae, Green fluorescent protein, Yeast-Cytology, Genetic recombination
While there have been many advances in fluorescence microscopy for single molecules in recent years, there are still limitations to studying protein activity and localization over time through fluorescence. It is difficult to distinguish between closelypacked fluorescent molecules, and impossible to determine the difference between proteins expressed at different times, and therefore observe differences in behavior over time. There is a need for a method of fluorescence that enables the study of the interactions of proteins throughout their lifetimes. This project aims to construct a system in S. cerevisiae for the expression of multifluorescent protein tags that vary in fluorescence color over time, enabling the differentiation of proteins expressed at different times.
Knobloch, Emmie Marie, "Clocking multifluorescent protein expression in yeast" (2017). Theses, Dissertations, and Projects. 1830.