Document Type

Article

Publication Date

7-1-2012

Publication Title

Systematic Biology

Abstract

The first analyses of gene sequence data indicated that the eukaryotic tree of life consisted of a long stem of microbial groups topped by a crown-containing plants, animals, and fungi and their microbial relatives. Although more recent multigene concatenated analyses have refined the relationships among the many branches of eukaryotes, the root of the eukaryotic tree of life has remained elusive. Inferring the root of extant eukaryotes is challenging because of the age of the group (∼1.7-2.1 billion years old), tremendous heterogeneity in rates of evolution among lineages, and lack of obvious outgroups for many genes. Here, we reconstruct a rooted phylogeny of extant eukaryotes based on minimizing the number of duplications and losses among a collection of gene trees. This approach does not require outgroup sequences or assumptions of orthology among sequences. We also explore the impact of taxon and gene sampling and assess support for alternative hypotheses for the root. Using 20 gene trees from 84 diverse eukaryotic lineages, this approach recovers robust eukaryotic clades and reveals evidence for a eukaryotic root that lies between the Opisthokonta (animals, fungi and their microbial relatives) and all remaining eukaryotes.

Keywords

Eukaryotes, gene tree, molecular systematics, species tree reconciliation, tree of life

Volume

61

Issue

4

First Page

653

Last Page

660

DOI

10.1093/sysbio/sys026

ISSN

10635157

Rights

© 2012 The Author(s).

Comments

Archived as published.

Included in

Biology Commons

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