Interaction-Dependent PCR: Identification of Ligand-Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment

Authors

Lynn M. McGregor, Harvard University
David J. Gorin, Harvard UniversityFollow
Christoph E. Dumelin, Harvard University
David R. Liu, Harvard University

Document Type

Article

Publication Date

11-10-2010

Publication Title

Journal of the American Chemical Society

Abstract

Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein-ligand pairs out of 67 599 possible sequences.

Volume

132

Issue

44

First Page

15522

Last Page

15524

DOI

10.1021/ja107677q

ISSN

00027863

Rights

© 2010 American Chemical Society

Comments

Archived as published. Open access article.

Recommended Citation

McGregor, Lynn M.; Gorin, David J.; Dumelin, Christoph E.; and Liu, David R., "Interaction-Dependent PCR: Identification of Ligand-Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment" (2010). Chemistry: Faculty Publications, Smith College, Northampton, MA.
https://scholarworks.smith.edu/chm_facpubs/63