Document Type
Article
Publication Date
11-2023
Publication Title
HardwareX
Abstract
Atmospheric Solids Analysis Probe (ASAP) mass spectrometry is a versatile technique allowing direct sampling of solid and liquid samples, but its adoption is limited due to the high cost of commercial ASAP systems. To address this, we present OpenASAP, an open-source ASAP system for mass spectrometers that can be fabricated for $20 or less using 3D-printing. Our design is readily adaptable to instruments from different manufacturers and can be produced with a variety of additive manufacturing techniques on consumer-grade 3D-printers. The probe allows for rapid sampling of solid and liquid samples without sample preparation, making it useful for high throughput screening, investigating spatial localization and function of analytes in biological samples, and incorporating mass spectrometry in instructional settings. We demonstrate its effectiveness by obtaining mass spectra of three natural product standards at levels as low as 10 ng/ml in liquid samples, and detecting these metabolites in microbial cultures that are difficult to analyze due to complex sample matrices or analyte properties. Furthermore, we demonstrate direct sampling of thin layer chromatography (TLC) spots of these cultures.
Keywords
Mass, Spectrometry, Atmospheric, Solids, Analysis, Probe, Ambient ionization
Volume
16
First Page
e00490
DOI
10.1016/j.ohx.2023.e00490
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Samples, Robert; Mukoyama, Rico; Shaffer, Jacob; Mikucki, Jill; and Giddings, Lesley Ann, "OpenASAP: An Affordable 3D Printed Atmospheric Solids Analysis Probe (ASAP) Mass Spectrometry System for Direct Analysis of Solid and Liquid Samples" (2023). Chemistry: Faculty Publications, Smith College, Northampton, MA.
https://scholarworks.smith.edu/chm_facpubs/95
Comments
Archived as published.